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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
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Dietary conjugated linoleic acids decrease leptin in porcine adipose tissue.

Alessia Di Giancamillo1, Raffaella Rossi, Francesca Vitari

  • 1Department of Veterinary Sciences and Technologies for Food Safety, University of Milan, 20133 Milan, Italy.

The Journal of Nutrition
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PubMed
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Dietary conjugated linoleic acids (CLA) supplementation in heavy pigs led to reduced leptin expression and increased adipocyte apoptosis in white adipose tissue (WAT). These changes suggest a potential increase in noradrenergic stimulation of lipolysis.

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Area of Science:

  • Animal Science
  • Nutritional Biochemistry
  • Adipose Tissue Biology

Background:

  • Conjugated linoleic acids (CLA) are fatty acids with potential metabolic effects.
  • White adipose tissue (WAT) plays a crucial role in energy homeostasis.
  • Understanding CLA's impact on WAT is important for animal nutrition and physiology.

Purpose of the Study:

  • To investigate the effects of dietary CLA on the characteristics of white adipose tissue (WAT) in heavy pigs.
  • To examine the influence of CLA on adipocyte size, proliferation, apoptosis, and leptin expression in WAT.
  • To explore the relationship between CLA, leptin, and noradrenergic activity in porcine WAT.

Main Methods:

  • Twelve heavy pigs were divided into two groups: control (CTR) and supplemented with 0.75% CLA.
  • Subcutaneous WAT was analyzed using chemical and microanatomical methods.
  • Immunohistochemistry and Western blot were used to quantify leptin expression; tyrosine hydroxylase activity was also measured.

Main Results:

  • CLA-fed pigs showed a trend towards higher protein content and smaller adipocytes in WAT.
  • Apoptosis of adipocytes was significantly greater in CLA-fed pigs compared to controls.
  • Leptin expression was significantly lower in adipocytes and WAT of CLA-fed pigs, while tyrosine hydroxylase activity was higher.

Conclusions:

  • Dietary CLA supplementation in heavy pigs influences WAT characteristics, including increased adipocyte apoptosis and reduced leptin expression.
  • Increased noradrenergic activity, indicated by higher tyrosine hydroxylase activity, may be linked to CLA's effects on leptin.
  • CLA's impact on WAT in heavy pigs suggests a complex interaction influencing lipolysis, despite not diminishing lipid content.