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Related Concept Videos

Proofreading01:43

Proofreading

Synthesis of new DNA molecules starts when DNA polymerase links nucleotides together in a sequence that is complementary to the template DNA strand. DNA polymerase has a higher affinity for the correct base to ensure fidelity in DNA replication. The DNA polymerase furthermore proofreads during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.Errors during Replication Are Corrected by the DNA Polymerase EnzymeGenomic DNA is synthesized in...
Proofreading01:31

Proofreading

Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase Enzyme
pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Guidelines for Writing Outcome01:11

Guidelines for Writing Outcome

When developing expected outcomes for a patient care plan, the nurse should adhere to the following recommendations:
Patient outcomes reflect the patient's response to the goal rather than what the nurse aims to achieve. Terminology should be observable and measurable to avoid the reader's interpretation. The desired outcome should be realistic and achievable in the designated care timeframe. Expected outcomes should align with adjunctive therapies. The outcome should enhance care evaluation by...
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
Preparation of 1° Amines: Hofmann and Curtius Rearrangement Mechanism01:26

Preparation of 1° Amines: Hofmann and Curtius Rearrangement Mechanism

The Hofmann and Curtius rearrangement reactions can be applied to synthesize primary amines from carboxylic acid derivatives such as amides and acyl azides. In the Hofmann rearrangement, a primary amide undergoes deprotonation in the presence of a base, followed by halogenation to generate an N-haloamide. A second proton abstraction produces a stabilized anionic species, which rearranges to an isocyanate intermediate via an alkyl group migration from the carbonyl carbon to the neighboring...

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Related Experiment Video

Updated: Jun 20, 2026

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

Preparing a manuscript for submission.

W C G Peh1, K H Ng

  • 1Singapore Medical Journal, 2 College Road, Singapore. smj.editor@sma.org.sg

Singapore Medical Journal
|August 28, 2009
PubMed
Summary

Preparing a scientific manuscript requires careful organization and clear writing. Adhering strictly to journal guidelines and seeking mentorship are crucial for successful publication.

Area of Science:

  • Scientific communication
  • Scholarly publishing

Background:

  • Manuscript preparation is the final stage of scientific research.
  • Journal acceptance signifies project completion.

Purpose of the Study:

  • To provide guidance on effective manuscript preparation for journal submission.
  • To emphasize the importance of clarity, structure, and adherence to author instructions.

Main Methods:

  • Organizing material to best showcase research findings.
  • Writing in a clear and concise style.
  • Selecting the appropriate journal and manuscript category.

Main Results:

  • Meticulous preparation increases the likelihood of acceptance.
  • Following "Instructions to Authors" is essential.

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TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
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Preparation of Retinal Samples for Volume Electron Microscopy
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Preparation of Retinal Samples for Volume Electron Microscopy

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Optimizing Sample Preparation for Cryogenic Electron Microscopy
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Optimizing Sample Preparation for Cryogenic Electron Microscopy

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  • Experienced mentorship significantly aids the process.

    • Conclusions:

    • Effective manuscript preparation is key to successful scientific publication.
    • Clarity, structure, and adherence to journal guidelines are paramount.