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Updated: Jun 20, 2026

Metagenomic Analysis of Silage
Published on: January 13, 2017
Novel genes retrieved from environmental DNA by polymerase chain reaction: current genome-walking techniques for
1Laboratory of Enzyme Technology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague 4, Czech Republic. kotik@biomed.cas.cz
Abstract:
Environmental DNA is an extremely rich source of genes encoding enzymes with novel biocatalytic activities. To tap this source, function-based and sequence-based strategies have been established to isolate, clone, and express these novel metagenome-derived genes. Sequence-based strategies, which rely on PCR with consensus primers and genome walking, represent an efficient and inexpensive alternative to activity-based screening of recombinant strains harbouring fragments of environmental DNA. This review covers the diverse array of genome-walking techniques, which were originally developed for genomic DNA and currently are also used for PCR-based recovery of entire genes from the metagenome. These sequence-based gene mining methods appear to offer a powerful tool for retrieving from the metagenome novel genes encoding biocatalysts with potential applications in biotechnology.
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