Related Experiment Video
Updated: Jun 20, 2026

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
Published on: September 13, 2014
A data base for polymer chromatography: temperature dependence of interaction parameters in liquid adsorption
Bernd Trathnigg1, Nguyen Viet Cuong, Hasnat Ahmed
1Institute of Chemistry, Karl-Franzens-University, Central Polymer Laboratory/Molecular Characteristics, Graz, Austria. bernd.trathnigg@uni-graz.at
Abstract:
The temperature dependence of retention behaviour of polyethylene glycol (PEG) and its mono- and dimethyl ethers was studied on various RP columns in different mobile phases. The accessible volumes and the interaction parameters were determined from slope and intercept in a plot of the elution volumes of the oligomers of a polymer homologous series as a function of the difference of the elution volumes of consecutive oligomers. A quite different dependence of the interaction parameters was observed in the different mobile phases. While in methanol-water the interaction parameter decreases with increasing temperature, the opposite effect is observed in acetonitrile (ACN)-water. In acetone-water, the temperature dependence is almost negligible.
More Related Videos
09:43Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method
Published on: April 11, 2020
11:38In situ FTIR Spectroscopy as a Tool for Investigation of Gas/Solid Interaction: Water-Enhanced CO2 Adsorption in UiO-66 Metal-Organic Framework
Published on: February 1, 2020
Related Concept Videos
Analyte Adsorption and Distribution
Adsorption Isotherms I
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Adsorption Isotherms II
Chromatographic Methods: Terminology