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Updated: Jun 20, 2026

Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
Published on: May 23, 2014
Proteomic characterization of midproliferative and midsecretory human endometrium
Jenny I C Chen1, Natalie J Hannan, Yunxian Mak
1Prince Henry's Institute of Medical Research, P.O. Box 5152, Clayton, Victoria 3168, Australia. jenny.chen@princehenrys.org
Abstract:
This study aimed to identify proteins differentially expressed in the human endometrium between the proliferative and secretory phases of normal menstrual cycles by 2D differential in-gel electrophoresis (DIGE). A total of 196 out of 1017 spots were differentially expressed (p < 0.05). Mass spectrometry identified 76 proteins representing 41 different gene products. Immunohistochemistry confirmed the observed changes in 3 representative proteins (Rho-GDIalpha, CLIC1, PGRMC1). Biological pathway analysis identified the Jnk and EGF signaling pathways as key regulators of protein expression in the midsecretory phase of endometrial proteome.
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