Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intermolecular Forces03:13

Intermolecular Forces

Atoms and molecules interact through bonds (or forces): intramolecular and intermolecular. The forces are electrostatic as they arise from interactions (attractive or repulsive) between charged species (permanent, partial, or temporary charges) and exist with varying strengths between ions, polar, nonpolar, and neutral molecules. The different types of intermolecular forces are ion–dipole, dipole–dipole, hydrogen bonds, and dispersion; among these, dipole–dipole, hydrogen bonds, and dispersion...
Micelles01:30

Micelles

Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...
Surface Active Agents01:27

Surface Active Agents

Surfactants, named for their behavior at interfaces, positively adsorb at the interfaces of two phases, reducing interfacial tension. Their versatility as emulsifiers, detergents, and foaming agents stems from this ability. Surfactants, often termed amphiphiles, share the property of amphipathy, with molecules having both hydrophilic and hydrophobic portions. The hydrophilic part is called the head, and the hydrophobic part, including an elongated alkyl substituent, forms the tail.Surfactants...
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Twin-compartment solid-liquid cells for neutron reflectometry.

Journal of applied crystallography·2026
Same author

How lipid composition shapes the nanostructural interaction of tumor biomarker alpha-fetoprotein and bovine serum albumin with model membranes.

Journal of colloid and interface science·2026
Same author

Molecular view of the interactions between the amphiphilic drug propranolol hydrochloride and model lipid membranes.

Journal of colloid and interface science·2026
Same author

Glycation Enhances Protein Association with Lipid Bilayer Membranes.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Designing biologically-relevant cell membrane models with natural lipid mixtures.

Biochimica et biophysica acta. Biomembranes·2025
Same author

Structural characterization of the lipid exchange between lipidic cubic phase nanoparticles and lipid monolayers using neutron reflectometry.

Journal of colloid and interface science·2025

Related Experiment Video

Updated: Jun 20, 2026

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering
09:45

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

Published on: March 17, 2023

Interaction of cationic lipoplexes with floating bilayers at the solid-liquid interface.

Jonathan P Talbot1, David J Barlow, M Jayne Lawrence

  • 1Institut Laue-Langevin, 6 rue Jules Horowitz, BP 156, F-38042 Grenoble, France.

Langmuir : the ACS Journal of Surfaces and Colloids
|August 29, 2009
PubMed
Summary
This summary is machine-generated.

Cationic lipoplexes destroy most model cell membranes, but bilayers on hydrocarbon layers remain intact. This suggests electrostatic interactions influence lipoplex-membrane disruption, highlighting a method for studying these interactions.

More Related Videos

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers
07:18

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

Published on: January 16, 2019

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Related Experiment Videos

Last Updated: Jun 20, 2026

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering
09:45

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

Published on: March 17, 2023

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers
07:18

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

Published on: January 16, 2019

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Area of Science:

  • Biophysics
  • Materials Science
  • Surface Chemistry

Background:

  • Lipoplexes, complexes of lipids and nucleic acids, are crucial for gene delivery.
  • Understanding lipoplex-membrane interactions is key to improving gene therapy efficacy.
  • Model membrane systems are essential for studying these complex interactions.

Purpose of the Study:

  • To investigate the interaction of cationic lipoplexes with various model membrane systems.
  • To determine how different interfacial layers affect lipoplex-induced membrane disruption.
  • To identify optimal model systems for studying lipoplex-membrane interactions.

Main Methods:

  • Neutron reflection was employed to analyze lipoplex interactions.
  • "Floating" phospholipid bilayers were prepared on silicon/water interfaces.
  • Model membranes were supported by phospholipid bilayers, polymer cushions, or hydrocarbon layers.

Main Results:

  • Cationic lipoplexes (DNA/DDAB with cholesterol or DOPE) destroyed three of four tested negatively charged bilayers.
  • Membrane destruction rate varied with the separating layer's composition.
  • Bilayers supported by a chemically grafted hydrocarbon layer remained intact.

Conclusions:

  • The underlying substrate layer significantly influences lipoplex-membrane interactions.
  • Electrostatic interactions, potentially involving the SiO2 surface, play a role in lipoplex binding and membrane disruption.
  • Floating bilayers on hydrocarbon layers provide a robust model for detailed lipoplex-membrane interaction studies.