Simultaneous determination of five benzimidazoles in feeds using high-performance capillary electrophoresis
Jianzhong Shen1, Jing Tong, Haiyang Jiang
1China Agricultural University, National Reference Laboratories for Veterinary Drug Residue, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Beijing 100193, People's Republic of China.
Abstract:
A high-performance capillary electrophoretic method for determination of five benzimidazoles (thiabendazole, albendazole, fenbendazole, mebendazole, oxfendazole) in animal feeds was developed. Separation was carried out in an 80.5 cm x 75 microm id fused-silica uncoated capillary and injected by hydrodynamic injection (35 mbar x 8 s) at a wavelength detection of 295 nm. The drugs were completely resolved in 50 mmol/L citric acid buffer (pH 1.9) with 30 kV at a temperature of 25 degrees C. Linear range was 1-80 microg/mL; limits of detection for the analytes were less than 2 mg/kg; the overall recoveries for benzimidazoles were >73%, with coefficients of variation below 10%.
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