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Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
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Methodology for determining disulfide linkage patterns of closely spaced cysteine residues

Bing Zhang1, Steven L Cockrill

  • 1Analytical Sciences, Amgen, Inc., 4000 Nelson Road, Longmont, Colorado 80503, USA.

Analytical Chemistry
|September 1, 2009
PubMed

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Sulfides are the sulfur analog of ethers, just as thiols are the sulfur analog of alcohol. Like ethers, sulfides also consist of two hydrocarbon groups bonded to the central sulfur atom. Depending upon the type of groups present, sulfides can be symmetrical or asymmetrical. Symmetrical sulfides can be prepared via an SN2 reaction between 2 equivalents of an alkyl halide and one equivalent of sodium sulfide.
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