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Modeling process-induced cell damage in the biodispensing process.

Minggan Li¹, Xiaoyu Tian, Ning Zhu

¹Department of Mechanical Engineering, University of Saskatchewan, Saskatoon, Canada.

Tissue Engineering. Part C, Methods

|September 1, 2009

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This study introduces a new method to quantify cell damage from mechanical forces during biodispensing. The model accurately predicts cell injury, crucial for optimizing biomanufacturing processes.

Area of Science:

Biomanufacturing
Cellular mechanics
Bioprocess engineering

Background:

Biomanufacturing increasingly uses living cells, requiring precise cell manipulation.
Biodispensing offers high efficiency but exposes cells to mechanical stress.
Quantifying cell damage in biodispensing is essential for process optimization.

Purpose of the Study:

To develop and validate a quantitative method for assessing force-induced cell damage during biodispensing.
To establish relationships between mechanical forces (hydrostatic pressure, shear stress) and cell damage.
To model and predict cell damage percentages in the biodispensing process.

Main Methods:

Developed cell damage laws linking mechanical stress to cellular injury.
Modeled the forces cells experience during biodispensing.

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Applied damage laws to simulation data to predict cell damage.

Validated the model using Schwann cells and 3T3 fibroblasts.

Main Results:

Successfully quantified force-induced cell damage in biodispensing.
Demonstrated a method to predict cell damage percentages.
Experimental and simulation results showed good agreement, validating the model's effectiveness.

Conclusions:

The presented method effectively quantifies cell damage in biodispensing.
This model is valuable for optimizing biomanufacturing processes involving cell manipulation.
Further research can refine this approach for various cell types and biodispensing parameters.