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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 20, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Reproducibility of quantitative RT-PCR array in miRNA expression profiling and comparison with microarray analysis.

Yongxin Chen1, Jonathan A L Gelfond, Linda M McManus

  • 1Department of Surgery, University of Texas Health Science Center, San Antonio, TX 78229, USA. cheny4@uthscsa.edu

BMC Genomics
|September 1, 2009
PubMed
Summary
This summary is machine-generated.

Quantitative RT-PCR arrays (qPCR-arrays) offer high reproducibility and sensitivity for microRNA (miRNA) profiling. This method demonstrates superior specificity and a lower false positive rate compared to microarrays, making it ideal for miRNA expression analysis.

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Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
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Last Updated: Jun 20, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

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Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 3, 2010

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) play crucial roles in biological processes.
  • Accurate miRNA profiling is essential for understanding normal and disease states.
  • High-throughput miRNA analysis faces challenges due to the increasing number of known miRNAs.

Purpose of the Study:

  • To evaluate the performance of a novel quantitative RT-PCR array (qPCR-array) for miRNA expression profiling.
  • To compare the qPCR-array method with oligonucleotide microchip (microarray) technology.
  • To assess the impact of cDNA pre-amplification on miRNA detection sensitivity and reproducibility.

Main Methods:

  • Utilized quantitative RT-PCR array (qPCR-array) for miRNA profiling.
  • Compared qPCR-array performance against miRNA detection using oligonucleotide microarrays.
  • Analyzed reproducibility using replicate samples and assessed variation across different experimental conditions.

Main Results:

  • qPCR-array demonstrated high reproducibility and maintained relative miRNA expression levels after pre-amplification.
  • Pre-amplification enhanced sensitivity and increased the number of detectable miRNAs.
  • Microarray analysis showed low correlation (r=-0.443) with qPCR-array and a higher false positive rate for differential miRNA expression.

Conclusions:

  • TaqMan qPCR-array exhibits high reproducibility and reliability.
  • cDNA pre-amplification improves miRNA detection sensitivity without introducing systemic bias.
  • qPCR-array offers superior sensitivity and specificity compared to microarray-based miRNA detection.