Expression and purification of the recombinant mustard trypsin inhibitor 2 (MTI2) in Escherichia coli
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Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
Published on: March 6, 2013
Alessandra Stefan1, Luisa Ugolini, Elena Martelli
1Department of Industrial Chemistry, University of Bologna, Italy.
Journal of Bioscience and Bioengineering
|September 1, 2009
Abstract:
The mustard trypsin inhibitor 2, MTI2, was expressed in Escherichia coli. A specific procedure for its production and purification is described. The recombinant protein was recovered by protein extraction from the insoluble fraction, then renatured and purified by ion exchange and gel filtration chromatography. Finally, the inhibitory activity against trypsin was also determined.
