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Updated: Jun 20, 2026

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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Delta-di-carboxybutyl phosphoramidate of 2'-deoxycytidine-5'-monophosphate as substrate for DNA polymerization by
Ivan Zlatev1, Anne Giraut, François Morvan
1Institut des Biomolécules Max Mousseron, UMR 5247, Université Montpellier 2, CC1704, Place E. Bataillon, 34095 Montpellier Cedex 5, France.
Bioorganic & Medicinal Chemistry
|September 1, 2009
Abstract:
The replacement of the pyrophosphate moiety of 2'-deoxynucleoside triphosphates by non natural delta-dicarboxylic butyl amino acid allows incorporation of natural 2'-deoxycytidine into DNA using HIV-1 reverse transcriptase (RT) as enzyme. In contrast, the 3'-deoxycytidine analogue was not a substrate of the HIV-1 RT.

