Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
Tight Junctions01:29

Tight Junctions

Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
Surface Membrane Barriers01:18

Surface Membrane Barriers

The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
The Proteasome01:13

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patient-derived organoids reveal ductal dysfunction and CFTR-modulator responses in chronic pancreatitis.

Cell stem cell·2026
Same author

Plasmin-mediated fibrinolysis is required for hematopoietic recovery after 5-FU-induced myeloablation.

Blood advances·2026
Same author

A Novel Patient-Derived Xenograft Model of Inflammatory Breast Cancer.

Research square·2026
Same author

Metastasis suppressing properties of the cell-surface anchored serine protease prostasin: new functional and mechanistic insights from breast cancer.

Oncogenesis·2026
Same author

Correction: The effectiveness of nano chemotherapeutic particles combined with mifepristone depends on the PR isoform ratio in preclinical models of breast cancer.

Oncotarget·2026
Same author

Clinical and biochemical characterization of amyotrophic lateral sclerosis in a CHCHD10 R15L family.

Brain : a journal of neurology·2026
Same journal

Lxrα Deficiency Primes Retinal Degeneration, but Aging Drives Disease Severity.

The American journal of pathology·2026
Same journal

MYC is functionally required in both normal and neoplastic Meibomian glands.

The American journal of pathology·2026
Same journal

Fumaric Acid Esters as Modulators of Ocular Inflammation and Angiogenesis.

The American journal of pathology·2026
Same journal

Krüppel-like factor 5 inhibition rescues cavernous nerve-injured erectile dysfunction by preventing phenotypic switch and mitochondrial dysfunction-dependent apoptosis in corpus cavernosum smooth muscle cells.

The American journal of pathology·2026
Same journal

Apolipoprotein E4 as a Modifier of Chemotherapy Response.

The American journal of pathology·2026
Same journal

Spatial Pathobiology in the Omics Era: Transforming Modern Pathology.

The American journal of pathology·2026
See all related articles

Related Experiment Video

Updated: Jun 20, 2026

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica
07:47

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

Published on: February 10, 2023

Epithelial integrity is maintained by a matriptase-dependent proteolytic pathway.

Karin List1, Peter Kosa, Roman Szabo

  • 1Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA.

The American Journal of Pathology
|September 1, 2009
PubMed
Summary
This summary is machine-generated.

Matriptase, a serine protease, is crucial for epithelial tissue maintenance. Deleting the St14 gene in mice led to organ dysfunction and epithelial breakdown, highlighting matriptase

More Related Videos

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance
07:32

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

Published on: December 3, 2020

Related Experiment Videos

Last Updated: Jun 20, 2026

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica
07:47

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

Published on: February 10, 2023

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance
07:32

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

Published on: December 3, 2020

Area of Science:

  • Epithelial biology
  • Protease function
  • Molecular genetics

Background:

  • Matriptase (a transmembrane serine protease) is vital for epidermal differentiation.
  • Constitutive expression in various epithelia suggests a broader role in epithelial homeostasis.

Purpose of the Study:

  • To investigate the essential role of matriptase in maintaining diverse epithelial tissues.
  • To generate and analyze a mouse model with conditional St14 gene deletion.

Main Methods:

  • Conditional deletion of the St14 gene (encoding matriptase) in mouse epithelial tissues.
  • Assessment of organ function, epithelial integrity, and tight junction complex in knockout models.

Main Results:

  • Ablation of St14 caused severe organ dysfunction across multiple epithelial types.
  • Increased epithelial permeability, loss of tight junction function, and protein mislocalization were observed.
  • Generalized epithelial demise occurred following St14 deletion.

Conclusions:

  • Matriptase is essential for the homeostasis of simple and stratified epithelia.
  • The St14 knockout mouse model is valuable for studying matriptase in physiological and pathological contexts.