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Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
Published on: July 15, 2021
Nanoscale 3D cellular imaging by axial scanning transmission electron tomography
Martin F Hohmann-Marriott1, Alioscka A Sousa, Afrouz A Azari
1National Institute of Biomedical Imaging and Bioengineering, Bethesda, Maryland, USA.
None:
Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context, but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We found that scanning transmission electron tomography of 1,000-nm-thick samples using axial detection provided resolution comparable to that of conventional electron tomography. We demonstrated the method by reconstructing a human erythrocyte infected with the malaria parasite Plasmodium falciparum.
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