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The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
Published on: February 27, 2026
Thrombin generation testing in haemophilia comprehensive care centres
1Unité d'Hémostase Clinique, Hôpital Edouard Herriot, Lyon, France. ydargaud@univ-lyon1.fr
Haemophilia : the Official Journal of the World Federation of Hemophilia
|September 2, 2009
Summary
Haemophilia comprehensive care centres (HCCCs) provide essential patient and professional education. New laboratory tools, like thrombin generation testing (TGT), are needed to better predict bleeding risk and treatment response in haemophilia patients.
Area of Science:
- Hematology
- Clinical Diagnostics
- Biochemistry
Background:
- Haemophilia comprehensive care centres (HCCCs) have evolved over 50 years.
- Initially focused on bleeding management education, HCCCs are now centres of excellence.
- They offer multidisciplinary care, conduct research, and contribute significantly to haemophilia management.
Purpose of the Study:
- To review unmet laboratory needs in haemophilia management.
- To explore the potential applications of thrombin generation testing (TGT) in haemophilia care.
- To discuss the technical and standardization challenges associated with TGT.
Main Methods:
- Review of existing literature on HCCCs and laboratory diagnostics in haemophilia.
- Analysis of the role of molecular biology in improving haemophilia diagnostics and treatment.
- Examination of the growing interest in thrombin generation testing (TGT) as a potential diagnostic tool.
Main Results:
- Standardization of factor VIII (FVIII) and FIX measurements and inhibitor detection has been achieved.
- Molecular biology has enhanced diagnostics and led to safer FVIII and FIX concentrates.
- Accurate phenotyping for bleeding risk and treatment response prediction remains a challenge.
Conclusions:
- Despite advancements, predicting individual bleeding risk and treatment response in haemophilia patients is difficult.
- Thrombin generation testing (TGT) shows promise as a new laboratory tool to assess overall coagulation capacity.
- Further research and standardization are needed to fully implement TGT in haemophilia management.
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