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Isolation of Human Islets from Partially Pancreatectomized Patients
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Validation of methodologies for quantifying isolated human islets: an Islet Cell Resources study.

H J Kissler1, J C Niland, B Olack

  • 1Department of Surgery, Northwestern University, Chicago, IL 60611, USA.

Clinical Transplantation
|September 2, 2009
PubMed
Summary

Accurate islet mass quantification is vital for successful islet transplantation. A new digital image analysis method proved more accurate and consistent than standard manual counting for human islet enumeration.

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Area of Science:

  • Endocrinology
  • Transplantation Biology
  • Medical Imaging

Background:

  • Islet mass quantification is critical for assessing islet product quality for type 1 diabetes transplantation.
  • Ensuring a potent islet graft is essential for therapeutic efficacy.

Purpose of the Study:

  • To validate the standard manual counting procedure for human islet quantification.
  • To evaluate a novel software-assisted digital image analysis method for islet quantification.

Main Methods:

  • Multi-center study involving eight institutions from the Islet Cell Resources Consortium.
  • Assessed accuracy, repeatability, and intermediate precision of the standard counting procedure.
  • Evaluated a supplemental digital image analysis method for islet quantification.

Main Results:

  • The standard counting procedure for isolated human islets was validated for accuracy, repeatability, and intermediate precision.
  • Software-assisted digital image analysis demonstrated superior accuracy and consistency compared to manual counting.

Conclusions:

  • Standard islet counting methods are valid but computer-assisted digital image analysis offers enhanced quality assurance.
  • Digital image analysis provides a permanent record, improving current good manufacturing practice for islet products.