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Updated: Jun 20, 2026
Exploring the Potential of Mesenchymal Stem Cell Sheet on The Development of Hepatocellular Carcinoma In Vivo
Published on: September 11, 2018
[Analysis on the expression of the stem cells related genes in hepatocellular carcinoma cell lines]
Can Zou1, Fang-jun Yuan, Wen-bo Zhou
1Institute of Liver Surgery, Dongfeng Hospital, Yunyang Medical College, Shiyan Hubei 442008, China.
Objective:
To investigate the expression of Oct4, Sox2, Nanog, SMO, beta-Catenin and Wnt5b mRNA in four hepatocellular carcinoma cell lines of SMMC-7721, Bel-7402, HepG2, MHCC-97 and normal hepatocellular cell line of L02, and to compare the response of these cell lines to all-trans retinoic acid.
Methods:
RT-PCR was used to detect expression of Oct4, Sox2, Nanog, SMO, beta-Catenin and Wnt5b mRNA in four hepatocellular carcinoma cell lines and normal hepatocellular cell line. Real time-PCR was used to quantify the expression of the genes.
Results:
There are different levels of expression of the stem cell-related gene in hepatocellular carcinoma cell lines and control cell line (P less than 0.05). There are significant differences in HepG2 and L-02 for the response to all-trans retinoic acid (P less than 0.05).
Conclusions:
The stem cell-related genes are differentially expressed in different hepatocellular carcinoma cell lines.
