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Updated: Jun 20, 2026

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017
Epigenetic modification of rhizobial genome is essential for efficient nodulation
Hiroyuki Ichida1, Katsuyoshi Yoneyama, Takato Koba
1Laboratory of Plant Pathology, School of Agriculture, Meiji University, Kawasaki, Kanagawa 214-8571, Japan. hi@isc.meiji.ac.jp
Abstract:
CcrM is one of the solitary bacterial DNA methyltransferases which does not have corresponding restriction enzymes. We established a stable ccrM-overexpressing mutant of Mesorhizobium loti, MlccrM-OX, and performed molecular and phenotypic characterization of this strain. In the M. loti MlccrM-OX infected plants, nodulation was apparently delayed at 7 days after inoculation (dai), however, the nodules that eventually formed on the MlccrM-OX roots showed nitrogen fixing ability by at least 21 dai. These results suggest that the initial morphogenic events were affected by ccrM-overexpression and that the correct pattern of DNA methylation of the bacterial genome is not essential for plant-microbe symbiosis, but are required for efficient nodulation.
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