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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

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Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
09:42

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

Capillary microextraction (CME) and its application to trace elements analysis and their speciation.

Bin Hu1, Fei Zheng, Man He

  • 1Department of Chemistry, Wuhan University, Wuhan 430072, China. binhu@whu.edu.cn

Analytica Chimica Acta
|September 2, 2009
PubMed
Summary
This summary is machine-generated.

Capillary microextraction (CME) is a solvent-free technique for analyzing trace elements in various samples. This review covers CME

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Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Materials Science

Background:

  • Capillary microextraction (CME) is a miniaturized, solvent-free sample preparation method.
  • CME is widely used for trace element analysis in environmental, biological, food, and pharmaceutical samples.
  • It is often hyphenated with various analytical instruments.

Purpose of the Study:

  • To review the fundamentals and recent advancements in Capillary Microextraction (CME).
  • To highlight the application of CME in trace and ultra-trace element analysis and speciation.
  • To summarize capillary materials and their preparation methods for CME.

Main Methods:

  • Discussion of theoretical basis of CME.
  • Overview of different extraction modes: packed, open-tubular, and monolithic CME.
  • Summary of existing coating/monolithic materials and their preparation.

Main Results:

  • CME offers a green and efficient approach for sample preparation.
  • Various materials and modes enhance CME's applicability for trace element analysis.
  • Detailed methods for material preparation are presented.

Conclusions:

  • CME is a versatile technique for trace element analysis and speciation.
  • Advancements in materials and modes continue to expand CME applications.
  • This review provides a comprehensive resource for CME researchers.