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Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System
Published on: January 31, 2018
Recombinant Mycobacterium bovis BCG
Reginaldo G Bastos1, Sibele Borsuk, Fabiana K Seixas
1Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA.
Vaccine
|September 2, 2009
Summary
Bacillus Calmette-Guerin (BCG) vaccines can be engineered to express foreign antigens, creating recombinant BCG. These modified vaccines show potential for broader applications beyond tuberculosis prevention.
Area of Science:
- Immunology
- Vaccinology
- Microbial Genetics
Background:
- Bacillus Calmette-Guerin (BCG) is an attenuated Mycobacterium bovis strain widely used for tuberculosis vaccination.
- BCG establishes persistent infections and elicits robust cellular and humoral immune responses.
- Its inherent adjuvanticity and established genetic systems enable its use as a vaccine vector.
Purpose of the Study:
- To review strategies for expressing foreign antigens in BCG.
- To discuss the immune responses induced by recombinant BCG constructs.
- To explore the potential of recombinant BCG as an immunomodulator and vaccine vector.
Main Methods:
- Review of scientific literature on recombinant BCG development.
- Analysis of genetic engineering techniques for heterologous antigen expression in BCG.
- Evaluation of immunological data from studies using recombinant BCG.
Main Results:
- Various strategies exist for controlled and stable expression of viral, bacterial, and parasite antigens in BCG.
- Recombinant BCG constructs elicit specific immune responses against the delivered antigens.
- BCG's capacity to express foreign antigens opens avenues for novel vaccine development.
Conclusions:
- Recombinant BCG represents a promising platform for developing new vaccines against infectious diseases.
- Further research into recombinant BCG holds potential for advanced immunomodulatory therapies.
- BCG's versatility as a vaccine vector warrants continued investigation for diverse applications.
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