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Updated: Jun 20, 2026

Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
Published on: August 24, 2015
Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways
Rie Hasebe1, Michihito Sasaki, Hirofumi Sawa
1Laboratory of Prion Diseases, Graduate School of Veterinary Medicine, Hokkaido University, West 9 North 18, Kita-ku, Sapporo 060-0818, Japan. r-hasebe@vetmed.hokudai.ac.jp
Abstract:
We investigated the mechanism by which equine herpesvirus-1 (EHV-1) enters primary cultured equine brain microvascular endothelial cells (EBMECs) and equine dermis (E. Derm) cells. EHV-1 colocalized with caveolin in EBMECs and the infection was greatly reduced by the expression of a dominant negative form of equine caveolin-1 (ecavY14F), suggesting that EHV-1 enters EBMECs via caveolar endocytosis. EHV-1 entry into E. Derm cells was significantly reduced by ATP depletion and treatments with lysosomotropic agents. Enveloped virions were detected from E. Derm cells by infectious virus recovery assay after viral internalization, suggesting that EHV-1 enters E. Derm cells via energy- and pH-dependent endocytosis. These results suggest that EHV-1 utilizes multiple endocytic pathways in different cell types to establish productive infection.
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