Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Relevance of Anti-PF4 Antibody Isotypes and Endogenous Glycosaminoglycans and their Relationship with Inflammatory Biomarkers in Pulmonary Embolism Patients.

Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·2022

Same author

Hybrid Multimodal Imaging Synthons for Chemoselective and Efficient Biomolecule Modification with Chelator and Near-Infrared Fluorescent Cyanine Dye.

Pharmaceuticals (Basel, Switzerland)·2020

Same author

Probing two PESIN-indocyanine-dye-conjugates: significance of the used fluorophore.

Journal of materials chemistry. B·2020

Same author

Fluorescent Chemosensors for Cu<sup>2+</sup> Ions: Fast, Selective, and Highly Sensitive.

Angewandte Chemie (International ed. in English)·2018

Same author

A Fluorescent Probe for Glycosaminoglycans Applied to the Detection of Dermatan Sulfate by a Mix-and-Read Assay.

Molecules (Basel, Switzerland)·2017

Same author

Accessing Structurally Diverse Near-Infrared Cyanine Dyes for Folate Receptor-Targeted Cancer Cell Staining.

Chemistry (Weinheim an der Bergstrasse, Germany)·2017

Search research articles

Related Experiment Video

Updated: Jun 20, 2026

Detection of Protease Activity by Fluorescent Peptide Zymography

Detection of Protease Activity by Fluorescent Peptide Zymography

Published on: January 20, 2019

Lanthanide-based fluorogenic peptide substrate for the highly sensitive detection of thermolysin.

Ann-Kathrin Marguerre¹, Roland Krämer

¹Anorganisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany.

Bioorganic & Medicinal Chemistry Letters

|September 2, 2009

Summary

Researchers developed a new, sensitive assay for detecting thermolysin, a zinc-dependent enzyme. This method uses a novel fluorogenic substrate and time-resolved fluorescence, achieving a 50 picomolar detection limit.

More Related Videos

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Published on: December 19, 2020

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Published on: January 16, 2019

Related Experiment Videos

Last Updated: Jun 20, 2026

Detection of Protease Activity by Fluorescent Peptide Zymography

Detection of Protease Activity by Fluorescent Peptide Zymography

Published on: January 20, 2019

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Published on: December 19, 2020

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Published on: January 16, 2019

Area of Science:

Biochemistry
Analytical Chemistry
Enzymology

Background:

Thermolysin is a crucial zinc-dependent metalloprotease.
Accurate detection of thermolysin is vital in various biological and industrial applications.
Existing detection methods may lack sensitivity or require complex procedures.

Purpose of the Study:

To develop a novel, highly sensitive assay for thermolysin detection.
To create a new fluorogenic substrate for thermolysin.
To utilize time-resolved fluorescence for enhanced detection.

Main Methods:

Synthesis of a novel fluorogenic, lanthanide-based oligopeptide substrate.
Development of a time-resolved fluorescence measurement assay.
Characterization of the assay's sensitivity and performance for thermolysin detection.

Main Results:

A new fluorogenic substrate specific for thermolysin was successfully synthesized.
A highly sensitive time-resolved fluorescence assay for thermolysin was established.
The assay demonstrated a low detection limit of 50 picomolar (3.5 femtomoles).

Conclusions:

The developed assay provides a sensitive and efficient method for thermolysin detection.
The novel substrate and time-resolved fluorescence approach offer advantages over existing techniques.
This assay has potential applications in research, diagnostics, and industrial processes requiring thermolysin quantification.