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Related Concept Videos

Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with cytoskeletal...
Structure of Lipids03:38

Structure of Lipids

Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic birds and...
Structure of Lipids03:38

Structure of Lipids

Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic birds and...
Structure of Lipids03:38

Structure of Lipids

Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic birds and...
Micelles01:30

Micelles

Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...
Assembly of the Lipid Bilayer in the ER01:28

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Updated: Jun 20, 2026

Realistic Membrane Modeling Using Complex Lipid Mixtures in Simulation Studies
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Realistic Membrane Modeling Using Complex Lipid Mixtures in Simulation Studies

Published on: September 1, 2023

[Elucidation of lipid complex formation mechanisms by static/dynamic structural evaluation].

Minoru Nakano1

  • 1Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan. mnakano@pharm.kyoto-u.ac.jp

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|September 2, 2009
PubMed
Summary

This study explores how lipid dynamics and membrane environments influence protein interactions, crucial for understanding biomembrane function. Small-angle neutron scattering (SANS) reveals lipid transfer protein activity and dynamics.

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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

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Realistic Membrane Modeling Using Complex Lipid Mixtures in Simulation Studies
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Realistic Membrane Modeling Using Complex Lipid Mixtures in Simulation Studies

Published on: September 1, 2023

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

Area of Science:

  • Biophysics
  • Membrane Biology
  • Biochemistry

Background:

  • Biomembranes are key sites for signal transduction, with protein-membrane interactions governed by the lipid environment.
  • Lipid dynamics, including interbilayer and transbilayer movement, are regulated by specific proteins and enzymes.
  • Understanding membrane structure and dynamics is vital for linking function, structure, and lipid behavior.

Purpose of the Study:

  • To investigate the production of lipid nanoparticles with nonlamellar liquid crystalline phases.
  • To examine membrane-protein interactions in the context of high-density lipoprotein biogenesis.
  • To characterize lipid transfer dynamics using small-angle neutron scattering (SANS).

Main Methods:

  • Production of lipid nanoparticles with nonlamellar liquid crystalline phases.
  • Analysis of membrane-protein interactions, specifically with apolipoprotein A-I (apoA-I).
  • Characterization of lipid transfer dynamics using small-angle neutron scattering (SANS).

Main Results:

  • Different phospholipids differentially affect membrane-apolipoprotein A-I (apoA-I) interactions, influencing discoidal lipid-protein complex formation.
  • Phosphatidylethanolamine enhances apoA-I binding by increasing hydration and acyl chain order.
  • Sphingomyelin and phosphatidylserine facilitate discoidal particle formation through altered membrane interfaces and pH-induced conformational changes in apoA-I.
  • SANS is validated as a method to determine membrane lipid dynamics and protein activity in lipid migration.

Conclusions:

  • Lipid composition significantly impacts protein-lipid complex formation, relevant to lipoprotein biogenesis.
  • SANS provides a powerful tool for studying lipid dynamics and protein involvement in lipid transport within membranes.