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Updated: Jun 20, 2026

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
10:18

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Published on: February 9, 2015

Generation of human PTH1R construct with FLAG epitope located internally: comparison of two-fragment assembly by

Bin Wang1, Yanmei Yang

  • 1Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA. biw5@pitt.edu

Journal of Biomolecular Techniques : JBT
|September 2, 2009
PubMed

Keywords:
ligationpolymerase chain reactionprotein expressiontype I PTH/PTH-related peptide receptor

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