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Updated: Jun 20, 2026

Determining Cell Number During Cell Culture using the Scepter Cell Counter

Determining Cell Number During Cell Culture using the Scepter Cell Counter

Published on: November 26, 2010

A cell number counting factor alters cell metabolism.

Wonhee Jang¹, Owen G Schwartz, Richard H Gomer

¹Department of Life Science; Dongguk University; Seoul, South Korea.

Communicative & Integrative Biology

|September 2, 2009

Summary

Organisms use a secreted counting factor (CF) to regulate cell group size by altering cell adhesion and motility. CF also impacts cell metabolism, affecting glucose, pyruvate, and ATP levels.

Keywords:

ATP Dictyostelium discoideum lactate oxygen consumption pyruvate size regulation

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Determining Cell Number During Cell Culture using the Scepter Cell Counter

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Published on: September 22, 2019

Area of Science:

Cellular biology
Developmental biology
Biochemistry

Background:

Organismal size regulation during development remains poorly understood.
Dictyostelium discoideum cells aggregate and use a secreted counting factor (CF) to sense population density.

Purpose of the Study:

To investigate how the secreted counting factor (CF) regulates cell group size.
To elucidate the impact of CF on fundamental cellular metabolic processes.

Main Methods:

Observation of Dictyostelium discoideum cell aggregation.
Measurement of extracellular CF concentrations.
Analysis of intracellular metabolite levels (e.g., glucose-6-phosphate, fructose-1,6-bisphosphate, pyruvate, lactate, ATP).
Assessment of oxygen consumption rates.

Main Results:

High extracellular CF concentrations trigger decreased cell-cell adhesion and increased motility, leading to group dispersal.
CF signaling reduces glucose-6-phosphatase activity, lowering intracellular glucose.
CF alters key glycolytic intermediates, including fructose-1,6-bisphosphate, glucose-6-phosphate, and fructose-6-phosphate.
CF influences pyruvate, lactate, ATP levels, and oxygen consumption, indicating broad metabolic regulation.

Conclusions:

The secreted counting factor (CF) is a crucial signal for regulating Dictyostelium discoideum cell group size.
CF plays a significant role in modulating cellular metabolism to coordinate developmental processes.
Understanding CF's metabolic effects provides insights into developmental size control mechanisms.