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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Jun 20, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
08:04

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

Published on: March 13, 2014

A MS data search method for improved 15N-labeled protein identification.

Yaoyang Zhang1, Christian Webhofer, Stefan Reckow

  • 1Max Planck Institute of Psychiatry, Proteomics and Biomarkers, Munich, Germany.

Proteomics
|September 2, 2009
PubMed
Summary
This summary is machine-generated.

Stable isotope labeling in proteomics improves biomarker discovery but faces challenges with incomplete labeling. This study introduces a new strategy to enhance (15)N-labeled protein identification and improve quantitative accuracy in mass spectrometry.

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Published on: February 27, 2020

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Last Updated: Jun 20, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
08:04

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

Published on: March 13, 2014

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

Area of Science:

  • Proteomics
  • Biomarker Discovery
  • Mass Spectrometry

Background:

  • Quantitative proteomics utilizing stable isotope labeling and mass spectrometry (MS) is crucial for biomarker discovery.
  • Stable isotope metabolic labeling offers high quantitative accuracy by enabling direct sample comparison.
  • Incomplete stable isotope incorporation in mammalian metabolic labeling hinders protein identification and quantification.

Purpose of the Study:

  • To develop a strategy for improving (15)N-labeled protein identification rates in quantitative proteomics.
  • To enhance the comprehensiveness and accuracy of relative peptide quantification workflows.

Main Methods:

  • Utilizing stable isotope metabolic labeling strategies in mammalian organisms.
  • Implementing a novel approach to improve the success rate of (15)N database searches.
  • Applying mass spectrometry for quantitative proteomic analysis.

Main Results:

  • Significantly increased the number of identified (15)N-labeled proteins.
  • Achieved more comprehensive and accurate relative peptide quantification.
  • Overcame limitations associated with incomplete stable isotope incorporation.

Conclusions:

  • The developed strategy effectively enhances protein identification in (15)N metabolic labeling experiments.
  • This approach leads to more reliable and accurate quantitative proteomics for biomarker discovery.
  • Improved methodology addresses a key challenge in stable isotope labeling for mammalian proteomics.