Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Harnessing CRISPR-Cas12 and Microfluidics Chips for Multiplex Respiratory Pathogens Diagnosis.

ACS sensors·2026
Same author

Smartphone-Based Microbubble-Linked Immunosorbent Assay Powered by Classification-Regression Integrated Deep Learning for Portable Quantitative Biomarker Analysis.

ACS nano·2026
Same author

Culture-Free Microfluidics for Ultra-Rapid Antimicrobial Susceptibility Testing with AI in Resource-Limited Settings.

Analytical chemistry·2026
Same author

Gravity-Driven Formation of Water-in-Wax Spheres for Efficient One-Pot CRISPR Diagnostics.

ACS nano·2026
Same author

AI-integrated smartphone platform enables POC dual-channel glucose monitoring with an indicator-free nanozyme gel kit.

Biosensors & bioelectronics·2026
Same author

High-throughput identification of endogenous biomolecular condensates and phase-separating proteins.

Nature protocols·2026

Related Experiment Video

Updated: Jun 20, 2026

Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions
06:56

Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions

Published on: October 10, 2013

Environmentally friendly surface modification of PDMS using PEG polymer brush.

Zhaowei Zhang1, Xiaojun Feng, Qingming Luo

  • 1The Key Laboratory of Biomedical Photonics of MOE-Hubei Bioinformatics and Molecular Imaging Key Laboratory-Division of Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Department of Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, P. R. China.

Electrophoresis
|September 2, 2009
PubMed
Summary
This summary is machine-generated.

This study introduces an eco-friendly surface modification for polydimethylsiloxane (PDMS) microchips using polyethylene glycol amine (PEG-NH2). This method effectively prevents protein adsorption and improves separation performance for over 30 days.

More Related Videos

Light-induced Patterning and Grafting for Slippery Surfaces based on Silane-coated Nanoporous Structures
07:23

Light-induced Patterning and Grafting for Slippery Surfaces based on Silane-coated Nanoporous Structures

Published on: November 14, 2025

Fabricating Reactive Surfaces with Brush-like and Crosslinked Films of Azlactone-Functionalized Block Co-Polymers
10:09

Fabricating Reactive Surfaces with Brush-like and Crosslinked Films of Azlactone-Functionalized Block Co-Polymers

Published on: June 30, 2018

Related Experiment Videos

Last Updated: Jun 20, 2026

Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions
06:56

Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions

Published on: October 10, 2013

Light-induced Patterning and Grafting for Slippery Surfaces based on Silane-coated Nanoporous Structures
07:23

Light-induced Patterning and Grafting for Slippery Surfaces based on Silane-coated Nanoporous Structures

Published on: November 14, 2025

Fabricating Reactive Surfaces with Brush-like and Crosslinked Films of Azlactone-Functionalized Block Co-Polymers
10:09

Fabricating Reactive Surfaces with Brush-like and Crosslinked Films of Azlactone-Functionalized Block Co-Polymers

Published on: June 30, 2018

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Polydimethylsiloxane (PDMS) microfluidic devices are widely used but suffer from protein adsorption, which compromises their performance.
  • Surface modification is crucial for preventing nonspecific protein binding and enhancing separation efficiency in microfluidic applications.

Purpose of the Study:

  • To develop an environmentally friendly surface modification strategy for PDMS microchips using polyethylene glycol amine (PEG-NH2).
  • To evaluate the effectiveness of the PEG-NH2 modification in preventing protein adsorption and improving separation performance.
  • To assess the long-term stability of the modified PDMS surface.

Main Methods:

  • Synthesized PEG-NH2 using a modified procedure.
  • Employed a two-step grafting method for PDMS microchip surface modification.
  • Verified surface modification using Fourier-transform infrared spectroscopy (FTIR) and contact angle measurements.
  • Assessed electroosmotic flow (EOF) and protein adsorption resistance.
  • Demonstrated separation of FITC-labeled amino acids and BSA.

Main Results:

  • Successful grafting of PEG-NH2 onto the PDMS surface was confirmed.
  • Significant EOF suppression and resistance to nonspecific protein adsorption were observed for over 30 days.
  • High repeatability and reproducibility in the separation of four FITC-labeled amino acids were achieved.
  • Markedly improved electrophoretic performance for BSA separation on PEG-modified PDMS microchips compared to unmodified ones.

Conclusions:

  • The developed PEG-NH2 surface modification strategy is effective in preventing protein adsorption and enhancing separation performance in PDMS microchips.
  • The modification provides long-term surface stability, making it suitable for various microfluidic applications.
  • This environmentally friendly approach offers a promising solution for advanced protein separations in microfluidic devices.