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CD Spectroscopy to Study DNA-Protein Interactions
06:48

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Published on: February 10, 2022

Experimental aspects of solid state circular dichroism.

Ettore Castiglioni1, Paolo Biscarini, Sergio Abbate

  • 1JASCO Corporation, Hachioji-shi, Tokyo 192-8537, Japan. ettore.castiglioni@jasco-europe.com

Chirality
|September 2, 2009
PubMed
Summary
This summary is machine-generated.

Solid-state circular dichroism (CD) offers solvent-free spectra for insoluble or processed samples. Careful selection of sampling techniques is crucial for obtaining accurate solid-state CD results.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy

Background:

  • Circular dichroism (CD) is a spectroscopic technique.
  • Solid-state CD is gaining interest due to specific experimental needs.

Purpose of the Study:

  • To explore the application of circular dichroism in the solid state.
  • To highlight the importance of sampling techniques in solid-state CD.

Main Methods:

  • Discussion of various sampling techniques for solid-state CD.
  • Analysis of factors influencing solid-state CD spectra.

Main Results:

  • Solid-state CD can provide spectra without solvents.
  • Insolubility or sample manipulation are key drivers for solid-state CD.
  • Sampling method directly impacts spectral quality.

Conclusions:

  • Solid-state circular dichroism is a valuable alternative when solution-state CD is not feasible.
  • Proper methodology in sampling is essential for reliable solid-state CD data.