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Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
Published on: December 17, 2015
Pathway analysis for intracellular Porphyromonas gingivalis using a strain ATCC 33277 specific database
Erik L Hendrickson1, Qiangwei Xia, Tiansong Wang
1Department of Chemical Engineering, Box 355014 University of Washington, Seattle, WA 98195, USA. elh@u.washington.edu
BMC Microbiology
|September 3, 2009
Summary
This study re-analyzed Porphyromonas gingivalis proteomic data using a specific genome annotation, revealing increased energy and protein synthesis machinery within host cells. These findings suggest a more nutrient-rich intracellular environment and potential virulence shifts in P. gingivalis.
Area of Science:
- Microbiology
- Proteomics
- Genomics
Background:
- Porphyromonas gingivalis is a key pathogen in periodontal disease.
- Previous studies used outdated genome annotations for proteomic analysis.
- Understanding virulence factor expression during host cell invasion is crucial.
Purpose of the Study:
- To re-analyze Porphyromonas gingivalis proteomic data using strain-specific genome annotation.
- To investigate differential protein abundance and metabolic pathway shifts during intracellular lifestyle.
- To improve proteome coverage and quantitative accuracy.
Main Methods:
- Whole-cell quantitative proteomic analysis of P. gingivalis ATCC 33277.
- Utilized updated genome annotation specific to strain ATCC 33277.
- Analyzed differential protein abundance based on metabolic pathways.
Main Results:
- Improved proteome coverage by 7% using ATCC 33277 annotation.
- Increased abundance of proteins involved in energy production (ATP) and translation/transcription machinery.
- Altered short-chain fatty acid production, with increased propionate and decreased butyrate.
Conclusions:
- Strain-specific annotation enhances proteomic analysis accuracy and coverage.
- Intracellular P. gingivalis exhibits increased energy metabolism and protein synthesis.
- Shifts in fatty acid production may contribute to P. gingivalis virulence within host cells.
