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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jun 20, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Real-time DNA microarray analysis.

Arjang Hassibi1, Haris Vikalo, José Luis Riechmann

  • 1Institute for Cellular and Molecular Biology, University of Texas at Austin, TX 78712, USA. arjang@mail.utexas.edu

Nucleic Acids Research
|September 3, 2009
PubMed
Summary
This summary is machine-generated.

This study introduces real-time DNA microarrays for enhanced quantification. The method uses hybridization kinetics to accurately measure analyte concentrations, improving upon conventional systems.

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Last Updated: Jun 20, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
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11:22

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Conventional DNA microarrays face limitations like probe saturation and signal variations.
  • Accurate quantification of target analytes is crucial for gene expression analysis.

Purpose of the Study:

  • To present a novel quantification method for affinity-based DNA microarrays.
  • To enhance the detection dynamic range and reliability of microarray measurements.
  • To enable real-time detection for gene expression DNA microarrays.

Main Methods:

  • Utilizing real-time measurements of hybridization kinetics for quantification.
  • Developing a method impervious to common microarray artifacts.
  • Employing a Förster Resonance Energy Transfer (FRET)-based assay for empirical validation.

Main Results:

  • Demonstrated that the time-constant of target capturing is inversely proportional to analyte concentration.
  • Established a new fundamental parameter for estimating analyte concentrations.
  • Validated the real-time detection capabilities in gene expression DNA microarrays.

Conclusions:

  • Real-time DNA microarrays offer enhanced dynamic range and accuracy.
  • The presented method overcomes limitations of conventional microarray systems.
  • This approach provides a robust platform for sensitive analyte quantification.