Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ex vivo drug screening and clustering of bladder cancers for pre-clinical treatment prediction.

Communications medicine·2026
Same author

Managing emergency department patients with potential medication-related harm: a qualitative study.

International journal of clinical pharmacy·2025
Same author

Antimicrobial Stewardship in German non-university hospitals: baseline status and impact of a multifaceted AMS intervention within the prospective ID ROLL OUT study.

Infection·2025
Same author

Steatotic liver disease is a marker of multimorbidity, not underlying cirrhosis, in older adults.

Npj gut and liver·2025
Same author

Phase transitions in random circuit sampling.

Nature·2024
Same author

Phase and amplitude responses for delay equations using harmonic balance.

Physical review. E·2024

Related Experiment Video

Updated: Jun 20, 2026

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
08:48

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

Flash-lamp-pumped Ti:Al(2)O(3) laser using fluorescent conversion.

P Lacovara, L Esterowitz, R Allen

    Optics Letters
    |September 3, 2009
    PubMed
    Summary
    This summary is machine-generated.

    We achieved efficient room-temperature laser operation using flash-lamp-pumped titanium-doped sapphire. This laser system offers a wide tuning range and high output energy, with potential for further optimization.

    More Related Videos

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
    10:21

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

    Published on: May 5, 2016

    Fluorescence detection methods for microfluidic droplet platforms
    14:16

    Fluorescence detection methods for microfluidic droplet platforms

    Published on: December 10, 2011

    Related Experiment Videos

    Last Updated: Jun 20, 2026

    Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
    08:48

    Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

    Published on: November 22, 2019

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
    10:21

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

    Published on: May 5, 2016

    Fluorescence detection methods for microfluidic droplet platforms
    14:16

    Fluorescence detection methods for microfluidic droplet platforms

    Published on: December 10, 2011

    Area of Science:

    • Laser Physics
    • Materials Science

    Background:

    • Titanium-doped sapphire is a well-known laser gain medium.
    • Achieving efficient room-temperature operation is desirable for practical applications.

    Purpose of the Study:

    • To demonstrate efficient room-temperature laser operation in flash-lamp-pumped titanium-doped sapphire.
    • To characterize the laser performance, including threshold, efficiency, output energy, and tuning range.

    Main Methods:

    • Utilized a flash-lamp-pumped configuration.
    • Employed titanium-doped sapphire as the gain medium.
    • Measured laser parameters such as threshold energy, slope efficiency, output energy, and wavelength tuning range.

    Main Results:

    • Achieved efficient room-temperature laser operation.
    • Observed a lasing threshold of 20 J.
    • Recorded a slope efficiency of 0.5%.
    • Generated output energy exceeding 300 mJ.
    • Demonstrated a tuning range of 720-920 nm.

    Conclusions:

    • Efficient room-temperature laser operation in flash-lamp-pumped titanium-doped sapphire has been successfully demonstrated.
    • The study provides key performance metrics for this laser system.
    • Potential methods for further improving laser threshold and slope efficiency are discussed.