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Transillumination fluorescence imaging in mice using biocompatible upconverting nanoparticles.

Claudio Vinegoni¹, Daniel Razansky, Scott A Hilderbrand

¹Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, 185 Cambridge Street, Boston, Massachusetts 02114, USA. cvinegoni@mgh.harvard.edu

|September 3, 2009

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This study introduces a new method for autofluorescence-free imaging using upconverting nanoparticles. This technique enables clear visualization of biological tissues and holds promise for advanced medical imaging applications.

Area of Science:

Biomedical Optics
Nanotechnology
Fluorescence Imaging

Background:

Turbid media like tissues scatter light, complicating optical imaging.
Upconversion fluorescence offers potential for deep tissue imaging.
Autofluorescence from biological samples can obscure signals.

Purpose of the Study:

To develop and validate a method for generating upconverting fluorescence signals in biological tissues.
To demonstrate autofluorescence-free transillumination imaging using upconverting nanoparticles.
To enable artifact-free whole-body visualization of optical molecular probes.

Main Methods:

Established and validated a three-point Green's function for photon propagation modeling.
Utilized biocompatible upconverting nanoparticles for imaging.

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Performed transillumination imaging experiments on mice.

Main Results:

Accurate modeling of photon propagation in turbid phantoms and tissues was achieved.
Demonstrated the first autofluorescence-free transillumination imaging in mice using upconverting nanoparticles.
Successfully visualized nanoparticles within biological samples without autofluorescence interference.

Conclusions:

The developed Green's function accurately models light propagation for upconversion imaging.
Autofluorescence-free imaging with upconverting nanoparticles is feasible in vivo.
This method offers significant potential for artifact-free whole-body optical molecular imaging.