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Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
Published on: May 1, 2015
Selective D2-40 lymphatic endothelium immunoreactivity in developing human fetal skin appendages
C Bellin1, E Fulcheri, M Rutigliani
1Department of Pediatrics, S. Martino Hospital, Genova, Italy. carlobellini@ospedale-gaslini.ge.it
Lymphology
|September 4, 2009
Summary
The D2-40 antibody shows unique immunoreactivity patterns in developing human fetal hair follicles. This study details the expression of podoplanin in fetal skin development.
Area of Science:
- Developmental Biology
- Immunohistochemistry
- Dermatology
Background:
- D2-40 is a monoclonal antibody targeting podoplanin, a 40,000 Da O-linked sialoglycoprotein.
- Its application is increasing, but data on human fetal development is limited.
Purpose of the Study:
- To investigate the D2-40 antibody immunoreactivity in developing cutaneous adnexa of human fetuses.
- To characterize the expression patterns of podoplanin during fetal skin development.
Main Methods:
- Retrospective analysis of 1,098 human fetal autopsies.
- Selection of 48 fetuses from 12 weeks gestation to term.
- Immunohistochemical evaluation of D2-40 expression in hair follicle gems.
Main Results:
- D2-40 immunoreactivity in hair follicle gems was negative from 12-15 weeks gestation.
- Positive immunoreactivity was observed between 16-20 weeks gestation.
- Expression became negative again from 21 weeks gestation to term and in adult controls.
Conclusions:
- This study is the first to report intense D2-40 immunoreactivity in the hair follicle gems of developing human skin.
- The dynamic expression pattern suggests a role for podoplanin in fetal hair follicle development.
- Further research is warranted to elucidate the specific function of podoplanin in this context.
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