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Updated: Jun 20, 2026
An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
Published on: December 17, 2018
Immunoassay for cadmium detection and quantification
Gong-Liang Liu1, Ju-Fang Wang, Zhi-Yong Li
1School of Biosciences & Bioengineering, South China University of Technology, Guangzhou 510006, Guangdong, China.
Objective:
To detect cadmium in environmental and food samples by graphite furnace atomic absorption spectroscopy (GFAAS) and inductively coupled plasma atomic emission spectroscopy (ICPAES).
Methods:
An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed based on a cadmium-specific monoclonal antibody. IC-ELISA for cadmium in environmental and food samples was evaluated.
Results:
IC-ELISA showed an IC50 of 45.6 microg/L with a detection limit of 1.95 microg/L for cadmium, and showed a mean recovery ranging 97.67%-107.08%. The coefficient of variations for intra- and interassay was 3.41%-6.61% and 4.70%-9.21%, respectively. The correlation coefficient between IC-ELISA and GFAAS was 0.998.
Conclusion:
IC-ELISA can detect and quantify cadmium residue in environmental or food samples.
