Related Experiment Video
Updated: Jun 20, 2026
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
Rapid detection of rpoB gene mutations in rif-resistant M. tuberculosis isolates by oligonucleotide microarray
Ai-Hua Sun1, Xing-Li Fan, Li-Wei Li
1Department of Basic Medicine, Zhejiang Medical College, Hangzhou 310053, Zhejiang, China.
Objective:
To detect the specific mutations in rpoB gene of Mycobacterium tuberculosis by oligonucleotide microarray.
Methods:
Four wild-type and 8 mutant probes were used to detect rifampin resistant strains. Target DNA of M. tuberculosis was amplified by PCR, hybridized and scanned. Direct sequencing was performed to verify the results of oligonucleotide microarray.
Results:
Of the 102 rifampin-resistant strains 98 (96.1%) had mutations in the rpoB genes.
Conclusion:
Oligonucleotide microarray with mutation-specific probes is a reliable and useful tool for the rapid and accurate diagnosis of rifampin resistance in M. tuberculosis isolates.
