Related Experiment Video
Updated: Jun 20, 2026

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking (TXL) for Myopia
Published on: January 6, 2018
Infrared-based third and second harmonic generation imaging of cornea
Szu-Yu Chen1, Han-Chieh Yu, I-Jong Wang
1National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Department of Electrical Engineering, No 1 Roosevelt Road Section 4, Taipei 10617, Taiwan.
Abstract:
The cornea functions as an optical lens and plays an important role in vision. For corneal diagnosis and treatment such as refractive surgery, a microscopic imaging system with a 3-D cellular resolution and retinal safety is strongly desired. Recently, confocal and multiphoton microscopies have been applied to corneal imaging with visible to near-infrared light sources. To increase retinal safety, an infrared light source is be needed. In this work, an infrared-based third and second harmonic generation microscopic study of mouse eyes is reported with approximately 700-mum penetrability and high cellular resolution. This study provides a critical reference for future development of infrared-based corneal imaging.
Related Concept Videos
Infrared (IR) Spectroscopy: Overview
Different compounds display unique properties due to their...
IR Spectrometers
IR Spectroscopy: Molecular Vibration Overview
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
IR Absorption Frequency: Hybridization
Among the sp, sp2, and sp3 hybridized orbitals, sp orbitals have the maximum s character (50%). Consequently, the electrons are held more closely to the nucleus, resulting in stronger and shorter C–H bonds that stretch at a...
IR Frequency Region: Fingerprint Region
The...
