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Updated: Jun 20, 2026

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates
Published on: May 14, 2019
[Plasma membrane-related Ca(2+)-ATPase-1 gene silencing promotes insulin secretion in islet beta cells NIT]
Yan-ping Fang1, Ai-min Ji, Yue-lian Yang
1Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Objective:
To assess the effect of RNA interference-mediated gene silencing of plasma membrane-related Ca(2+)-ATPase-1 (PMR1) gene on the insulin secretion in islet beta cells NIT-1 in vitro.
Methods:
A small interfering RNA duplex (siPMR1) corresponding to the nucleotides 337-357 of mouse PMR1 cDNA was introduced into NIT-1 cells via liposomes. The gene silencing effect was assessed by RT-PCR, and the total insulin level in the transfected cells was measured by radioimmunoassay.
Results:
Transfection with siPMR1 resulted in obviously reduced PMR1 expression and increased insulin secretion in NIT-1 cells.
Conclusion:
The synthesized siPMR1 can significantly silence the expression of PMR1 and promote the secretion of insulin in the islet cells in vitro, which shed light on further studies of RNAi-based therapy of diabetes.
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