Related Experiment Video
Updated: Jun 20, 2026

Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection
Published on: May 10, 2024
[Expression of survivin gene in a rat model of acute pancreatitis]
Zhen-Dong Li1, Qing-Yong Ma, Yu-Hong Luo
1Department of Hepatobiliary Surgery, First Affiliated Hospital of Ji'nan University, Guangzhou, China. victor7922@163.com
Objective:
To investigate the expression of survivin in acute pancreatitis in rats.
Methods:
Acute pancreatitis was induced in rats by retrograde injection of sodium taurocholate into the pancreaticobiliary duct. The expressions of survivin in the pancreatic tissues was detected by immunohistochemisty, Western blotting and RT-PCR, and the apoptotic ratio of the acinar cells was determined by TUNEL assay.
Results:
Survivin was not detected in the control group, and in rat models of acute pancreatitis, the expressions of survivin protein and mRNA increased but the apoptotic index of the acinar cells decreased gradually with the severity of inflammation.
Conclusion:
Survivin is involved in the regulation of acinar cell apoptosis and also the necrosis of the apoptotic acinar cells through its anti-apoptosis activity to aggravate acute pancreatitis, suggesting its value as a promising marker in predicting the severity of acute pancreatitis.
Related Concept Videos
Acute Pancreatitis II: Pathophysiology
Acute Pancreatitis I: Introduction
Acute pancreatitis is characterized by rapid inflammation of the pancreas, often caused by factors like gallstone blockage or excessive alcohol consumption. Chronic pancreatitis, on the other hand, is a slow, progressive inflammation that may result from long-term alcohol abuse, obstructions in the pancreatic duct, or genetic factors.
The causes of acute pancreatitis include:
Acute Pancreatitis I: Introduction
Chronic Pancreatitis II: Pathophysiology

