Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hepatitis01:25

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intra-host viral population dynamics during acute hepatitis E virus infection.

mBio·2026
Same author

Exploration of the relationship between laboratory stress parameters and leukocyte coping capacity in ponies exposed to repetitive handling during a research project.

BMC veterinary research·2026
Same author

Simulated surface and toilet contamination during norovirus gastroenteritis: the role of toilet flushing, murine norovirus persistence, and inactivation by household and toilet cleaners.

The Journal of hospital infection·2026
Same author

Clinical and genomic profiling of <i>Klebsiella pneumoniae</i> in liver abscesses.

Virulence·2026
Same author

Development of a CRISPR-Cas13-based antiviral strategy against hepatitis E virus.

JHEP reports : innovation in hepatology·2026
Same author

Comment on a published new method in virucidal testing - T-25 method.

GMS hygiene and infection control·2026

Related Experiment Video

Updated: Jun 20, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
13:04

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

Characterization of determinants important for hepatitis C virus p7 function in morphogenesis by using

Christiane Brohm1, Eike Steinmann, Martina Friesland

  • 1Department of Molecular Virology, University Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany.

Journal of Virology
|September 4, 2009
PubMed
Summary
This summary is machine-generated.

Hepatitis C virus p7 protein is essential for producing infectious virions. A new assay shows p7 functions independently of viral processing, highlighting its role in virus assembly and release.

More Related Videos

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
16:49

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Published on: July 16, 2012

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
11:34

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

Published on: May 10, 2022

Related Experiment Videos

Last Updated: Jun 20, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
13:04

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
16:49

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Published on: July 16, 2012

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
11:34

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

Published on: May 10, 2022

Area of Science:

  • Virology
  • Molecular Biology
  • Membrane Protein Function

Background:

  • Hepatitis C virus (HCV) p7 is an integral membrane protein and a viroporin.
  • p7 forms ion channels crucial for viral assembly and release.
  • The exact role of p7 in virus production, separate from polyprotein processing, was unclear.

Purpose of the Study:

  • To develop a method to study HCV p7 function independently of polyprotein processing.
  • To determine if p7's ion channel activity or processing site modulation affects virus production efficiency.
  • To investigate the essentiality of p7 in the context of the viral lifecycle.

Main Methods:

  • Developed a trans-complementation assay to analyze p7 outside the HCV polyprotein.
  • Used transient and stable cell lines to rescue p7-defective HCV genomes.
  • Tested heterologous viroporins (Influenza A M2, HIV-1 Vpu) for complementation ability.

Main Results:

  • HCV p7 is absolutely essential for producing infectious HCV particles.
  • p7 can function independently of its upstream signal sequence and polyprotein processing.
  • A specific tyrosine residue near p7's dibasic motif is important for genotype 2a virus production.
  • Influenza A M2 and HIV-1 Vpu could not compensate for defective p7.

Conclusions:

  • HCV p7 is indispensable for infectious virion production.
  • The developed assay allows studying p7 structure-function relationships without confounding processing effects.
  • Further investigation into p7's determinants is facilitated by this novel experimental system.