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Updated: Jun 20, 2026
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
Published on: October 16, 2018
Molecular characterization of a full-length genome of a HIV-2 isolate from India
Susmita R Gurjar1, A Mangaiarkarasi, V Ravi
1Department of Neurovirology, National Institute of Mental Health and Neurosciences, Bangalore, India.
Background:
India accounts for 95% of HIV-2 infections in Asia. Molecular epidemiological studies have been performed on HIV-2 partial envelope gene and LTR sequences from isolates in India. Although, a single full-length sequence of an isolate from India is available in the GenBank, a comprehensive molecular analysis of a large number of HIV-2 isolates would be essential for understanding the genomic heterogeneity of HIV-2 isolates prevalent in the country.
Methods:
In this study, we determined the complete nucleotide and deduced amino acid sequence of a HIV-2 isolate that was obtained by coculture of infected cells. An extensive full-length genome analysis was performed with sequences available from the GenBank.
Results:
The isolate belonged to HIV-2 subtype A. Comparison of the genomic sequence with those of the 18 full-length sequences obtained from the GenBank showed the genetic distance ranging from 7.8% with CRIK (subtype A, India) to 30% with EHO (subtype B, Cote d'ivoire).
Conclusions:
Phylogenetic analysis indicated that this isolate exhibited close homology with CRIK. Analysis of the gene sequences indicated presence of 2 mutations associated with drug resistance to nucleoside reverse transcriptase inhibitors. Mutations associated with protease inhibitors were not observed in the isolate.
