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Functional analysis of the Autographa californica nucleopolyhedrovirus IAP1 and IAP2

XianDong Zeng1, Fang Nan, ChangYong Liang

  • 1Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.

Science in China. Series C, Life Sciences
|September 4, 2009
PubMed

Insights

Autographa californica nucleopolyhedrovirus (AcMNPV) genes iap1 and iap2 suppress apoptosis in insect cells. These viral genes are potential host range factors, influencing viral replication and host cell survival during infection.

Area of Science:

  • Virology
  • Molecular Biology
  • Insect Pathology

Background:

  • Autographa californica nucleopolyhedrovirus (AcMNPV) possesses three known apoptosis suppressor genes: p35, iap1, and iap2.
  • While AcMNPV P35 is a known pancaspase inhibitor, the specific roles of IAP1 and IAP2 in apoptosis suppression remain incompletely understood.

Purpose of the Study:

  • To elucidate the functional roles of AcMNPV IAP1 and IAP2 in apoptosis suppression.
  • To investigate the impact of these genes on viral replication and budded virus (BV) production during Helicoverpa armigera single nucleocapsid NPV (HearNPV) infection.

Main Methods:

  • Analysis of AcMNPV with a p35 deletion in BTI-Tn-5B1-4 (Tn-Hi5) cells infected with HearNPV.
  • Transient-expression assays to assess apoptosis suppression by IAP1 and IAP2.
  • Construction and analysis of recombinant HearNPVs expressing AcMNPV iap1, iap2, and p35 genes.

Main Results:

  • AcMNPV lacking p35 inhibited HearNPV-induced apoptosis and rescued HearNPV replication and BV production in Tn-Hi5 cells.
  • Both IAP1 and IAP2 were found to suppress apoptosis in Tn-Hi5 cells during HearNPV infection.
  • Recombinant HearNPVs expressing AcMNPV iap1, iap2, or p35 prevented apoptosis and supported HearNPV replication, but did not rescue BV production.

Conclusions:

  • AcMNPV iap1 and iap2 function independently as apoptosis inhibitors.
  • These viral genes are identified as potential host range factors, influencing viral interactions with host cells.

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