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Role of protein tyrosine nitration in neurodegenerative diseases and atherosclerosis

Jung Rok Lee1, Jae Kyung Kim, Soo Jae Lee

  • 1Department of Molecular Biotechnology, Konkuk University, Seoul, Korea.

Archives of Pharmacal Research
|September 4, 2009
PubMed

Insights

Nitric oxide creates reactive species like peroxynitrite, causing protein damage implicated in diseases. This review explores the role of protein nitration in neurodegenerative conditions and atherosclerosis.

Area of Science:

  • Biochemistry
  • Pathobiology
  • Molecular Biology

Background:

  • Nitric oxide (NO) produces reactive species, notably peroxynitrite (ONOO(-)).
  • Peroxynitrite mediates protein tyrosine nitration, altering protein structure and function.
  • Protein nitration is increasingly recognized as a significant posttranslational modification in disease.

Purpose of the Study:

  • To review the pathobiological roles of protein tyrosine nitration.
  • To highlight the involvement of peroxynitrite-mediated nitration in disease pathogenesis.
  • To discuss the implications for neurodegenerative diseases and atherosclerosis.

Main Methods:

  • Proteomics techniques, including mass spectrometry.
  • Gel electrophoresis methods like liquid and 2-D PAGE.
  • Immunodetection assays for identifying nitrated proteins.

Main Results:

  • Protein tyrosine nitration is a key pathogenic modification.
  • Nitrated proteins are found in pathological samples from affected tissues.
  • Identification of specific nitrated proteins provides insights into disease mechanisms.

Conclusions:

  • Protein nitration contributes to the pathophysiology of neurodegenerative diseases.
  • Protein nitration plays a role in the development of atherosclerosis.
  • Understanding protein nitration is crucial for developing therapeutic strategies.

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