Related Experiment Video
Updated: Jun 20, 2026

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
Published on: February 25, 2021
Glycosaminoglycan metabolism before molecular biology: reminiscences of our early work
1Veterans Affairs Medical Center, Bedford, MA, USA. jesilbert@aol.com
Abstract:
This article concerns personal reminiscences of research on proteoglycans accomplished by Jeremiah Silbert and his co-investigators over a 25-30 year period beginning in 1961. Radiolabeled substrates were prepared and incubated with subcellular particles from mast cells and cartilage to determine pathways and organization of heparin and chondroitin glycosaminoglycan formation together with sulfation. Microsomal/Golgi fractions were examined for localization and organization of synthesis. Cell surface heparan sulfate and chondroitin were examined for preliminary information regarding potential function, and techniques were developed to alter sulfation processes.
Related Concept Videos
Glycosaminoglycans
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...
Proteoglycans
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Protein Glycosylation
Glycosylation occurs in...
Biosynthesis of Polysaccharides
Matrix Proteoglycans and Glycoproteins
