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Updated: Jun 20, 2026
The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
Published on: August 4, 2018
A new pathogenesis-related protein, LrPR4, from Lycoris radiata, and its antifungal activity against Magnaporthe
Xiaodan Li1, Bing Xia, Yumei Jiang
1Qian Hu Hou Cun 1#, Jiangsu Province Key Laboratory for Plant Ex-Situ Conservation, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, 210014 Nanjing, People's Republic of China.
Abstract:
A new Lycoris radiata pathogenesis-related (PR)-4 gene, LrPR4 was isolated. LrPR4 encodes a 142 amino acid protein with a predicted molecular mass of 15.43 kDa and pI of 7.56. The putative LrPR4 shows high similarity to PR4 type proteins from various plant species and belongs to the Barwin family. Like other PR4s from monocot plants, LrPR4 protein contains a conserved Barwin domain and has a signal peptide at its N-terminus. The recombinant LrPR4 protein expressed in Escherichia coli showed activity towards hydrolysing RNA from L. radiata bulbs and antifungal activity. The results of this study suggest that LrPR4 may play a role in the disease resistance responses of plant against pathogen attacks though its antifungal activity.
Insights
A novel Lycoris radiata pathogenesis-related (PR)-4 gene, LrPR4, was identified. This gene exhibits RNA hydrolysis and antifungal activity, suggesting a role in plant disease resistance.
Area of Science:
- Plant molecular biology
- Biochemistry
- Plant pathology
Background:
- Pathogenesis-related (PR) proteins are crucial in plant defense mechanisms.
- PR-4 proteins, belonging to the Barwin family, are implicated in plant immunity.
- Lycoris radiata, a significant ornamental plant, requires further investigation into its defense systems.
Purpose of the Study:
- To isolate and characterize a novel PR-4 gene from Lycoris radiata.
- To investigate the biochemical and functional properties of the isolated LrPR4 protein.
- To elucidate the potential role of LrPR4 in Lycoris radiata's disease resistance.
Main Methods:
- Gene isolation and sequencing of LrPR4 from Lycoris radiata.
- Bioinformatic analysis for protein structure prediction (molecular mass, pI, conserved domains).
- Recombinant protein expression in Escherichia coli and in vitro functional assays (RNA hydrolysis, antifungal activity).
Main Results:
- A new Lycoris radiata pathogenesis-related (PR)-4 gene, LrPR4, was successfully isolated.
- The LrPR4 protein, with a conserved Barwin domain and signal peptide, showed similarity to other plant PR4s.
- Recombinant LrPR4 demonstrated RNA hydrolyzing activity and significant antifungal properties.
Conclusions:
- The LrPR4 gene encodes a functional PR-4 protein with potential roles in plant defense.
- LrPR4's demonstrated antifungal activity suggests its involvement in protecting Lycoris radiata against pathogens.
- This study provides insights into the molecular mechanisms of disease resistance in Lycoris radiata.
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