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Related Experiment Video

Updated: Jun 20, 2026

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
09:47

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

Published on: May 25, 2018

Methods for the proteomic identification of protease substrates.

Nicholas J Agard1, James A Wells

  • 1Department of Pharmaceutical Chemistry, University of California, San Francisco, UCSF MC 2552, 1700 4th St, San Francisco, CA 94158, United States of America.

Current Opinion in Chemical Biology
|September 5, 2009
PubMed
Summary

Degradomics offers unbiased methods to track protein cleavage, aiding research in cell death and immune function. These techniques identify protease substrates and cleavage sites, revealing over 300 caspase targets in apoptosis.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cellular Biology

Background:

  • Proteolysis is a crucial post-translational modification regulating key cellular processes like apoptosis, immunity, and development.
  • Understanding protease activity is vital for assessing downstream cellular consequences.
  • The field of degradomics focuses on tracking proteolytic events and identifying protease substrates.

Purpose of the Study:

  • To review unbiased methods for identifying protease substrates.
  • To summarize techniques for tracking the extent of protein cleavage.
  • To highlight the applications of degradomics in biological research.

Main Methods:

  • One-dimensional and two-dimensional gel-based approaches for substrate identification.
  • N-terminal peptide identification for simultaneous substrate and cleavage site determination.

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Last Updated: Jun 20, 2026

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
09:47

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

Published on: May 25, 2018

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
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Published on: February 21, 2019

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
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Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Published on: January 16, 2019

  • Quantitative methods for measuring cleavage events in endogenous proteolysis.

    • Main Results:

    • Degradomics encompasses unbiased methods for identifying protease substrates and cleavage sites.
    • Various techniques allow for the tracking and quantification of proteolytic events.
    • Over 300 caspase-cleaved substrates have been identified during apoptosis using these methods.

    Conclusions:

    • Degradomics provides powerful tools for studying proteolysis.
    • These methods have broad applications in understanding cellular processes regulated by proteases.
    • The identification of numerous caspase targets highlights the potential of degradomics in apoptosis research.