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Related Experiment Video

Updated: Jun 20, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Towards evolving a better repressor.

Robert Daber¹, Mitchell Lewis

¹Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6059, USA.

Protein Engineering, Design & Selection : PEDS

|September 5, 2009

Summary

Researchers uncovered new details about the lac operon, revealing the operator DNA

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Transcriptional regulation is crucial for metabolic pathways.
Epigenetic switches, formed by protein-operator DNA interactions, control gene expression.
The lac operon is a classical model for studying gene regulation.

Purpose of the Study:

To investigate novel aspects of the lac operon for improved epigenetic switch functionality.
To understand the relationship between operator binding affinity and gene inducibility.
To explore the role of operator DNA in determining binding affinity, specificity, and translational efficiency.

Main Methods:

Analysis of the lac operon's regulatory mechanisms.
Investigating the interaction between regulatory proteins and operator DNA sequences.

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Last Updated: Jun 20, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Published on: September 13, 2024

Assessing the impact of operator DNA properties on gene expression.

Main Results:

A direct relationship was found between operator binding affinity and gene inducibility.
Operator DNA is an active component, not passive, in genetic switches.
Operator DNA influences binding affinity, specificity, and translational efficiency.
Operator directionality can indirectly impact gene expression.

Conclusions:

Optimizing multiple factors of the operator DNA is essential for designing more effective epigenetic switches.
Understanding the fundamental properties of the lac operon provides insights into gene regulation.
Novel facets of the lac operon contribute to its optimal functionality.