Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Efficacy and safety analysis of venetoclax in combination with multidrug chemotherapy in patients with newly diagnosed acute leukemia of ambiguous lineage].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2025
Same author

[Efficacy and safety of gilteritinib combined with chemotherapy in newly diagnosed FLT3-mutated acute myeloid leukemia].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2025
Same author

[Long-term hypomethylating agents in patients with myelodysplastic syndromes: a multi-center retrospective study].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2024
Same author

[Clinical study of induction chemotherapy followed by allogeneic hematopoietic stem cell transplantation in the treatment of FLT3-ITD(+) acute myeloid leukemia with normal karyotype].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2023
Same author

[Estradiol regulates the expression of plasma membrane Ca<sup>2+</sup>-ATPase isoform 2 in inner ear of rats].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2023
Same author

[The role and mechanism of lncRNA C9ORF139 targeting miR-24-3P/TAOK1 in regulating the proliferation of acute myeloid leukemia cells].

Zhonghua yi xue za zhi·2022

Related Experiment Video

Updated: Jun 20, 2026

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Coherent Raman mixing and coherent anti-Stokes Raman scattering from individual micrometer-size droplets.

S X Qian, J B Snow, R K Chang

    Optics Letters
    |September 5, 2009
    PubMed
    Summary

    Coherent Raman mixing spectra reveal morphology-dependent peaks in ethanol and water droplets, unlike coherent anti-Stokes Raman scattering spectra. Spatial overlap of droplet conditions explains these distinct spectral features.

    More Related Videos

    Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)
    12:56

    Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)

    Published on: October 17, 2010

    Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
    12:54

    Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

    Published on: July 17, 2016

    Related Experiment Videos

    Last Updated: Jun 20, 2026

    Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
    09:46

    Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

    Published on: April 28, 2022

    Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)
    12:56

    Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)

    Published on: October 17, 2010

    Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
    12:54

    Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

    Published on: July 17, 2016

    Area of Science:

    • Spectroscopy
    • Physical Chemistry
    • Optical Physics

    Background:

    • Morphology-dependent resonances (MDRs) are optical phenomena observed in microdroplets.
    • Coherent Raman scattering techniques probe molecular vibrations.
    • Understanding droplet-based spectroscopy is crucial for various applications.

    Purpose of the Study:

    • To investigate the presence of morphology-dependent resonances in coherent Raman mixing spectra of microdroplets.
    • To compare these findings with coherent anti-Stokes Raman scattering (CARS) spectra.
    • To elucidate the underlying physical mechanisms responsible for observed spectral differences.

    Main Methods:

    • Acquisition of coherent Raman mixing spectra from individual micrometer-sized ethanol and water droplets.
    • Acquisition of coherent anti-Stokes Raman scattering (CARS) spectra from similar droplets.
    • Analysis of spectral features in relation to droplet morphology and optical conditions.

    Main Results:

    • Coherent Raman mixing spectra exhibited regularly spaced peaks attributed to MDRs of spherical droplets.
    • Coherent anti-Stokes Raman scattering (CARS) spectra showed no evidence of morphology-dependent peaks.
    • The spatial overlap of phase-matching conditions within the droplet was identified as the key factor explaining the spectral differences.

    Conclusions:

    • Morphology-dependent resonances are observable in coherent Raman mixing spectra of microdroplets.
    • The absence of MDRs in CARS spectra is linked to specific spatial overlap requirements.
    • This study highlights the importance of considering droplet geometry and optical coupling in Raman spectroscopy.