Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Imaging features of posterior polymorphous corneal dystrophy observed by <i>in vivo</i> confocal microscopy].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2022
Same author

[Descemet membrane endothelial keratoplasty in initial cases: analysis and management of surgical complications].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2021
Same author

Underwater spatially, spectrally, and temporally resolved optical monitoring of aquatic fauna.

Optics express·2020
Same author

[The 8-year follow-up study for clinical diagnostic potentials of frequency-doubling technology perimetry for perimetrically normal eyes of open-angle glaucoma patients with unilateral visual field loss].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2018
Same author

ATP2C1 gene mutations in Hailey-Hailey disease and possible roles of SPCA1 isoforms in membrane trafficking.

Cell death & disease·2016
Same author

The Warburg effect: a balance of flux analysis.

Metabolomics : Official journal of the Metabolomic Society·2015
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: Jun 20, 2026

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Laser spectroscopy using beam-overlap modulation.

T P Duffey, D Kammen, A L Schawlow

    Optics Letters
    |September 5, 2009
    PubMed
    Summary
    This summary is machine-generated.

    A novel Doppler-free laser spectroscopy technique uses laser beam position modulation for precise measurements. This method achieves background-free spectra in fluorescence experiments without complex intermodulation techniques.

    More Related Videos

    An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers
    09:49

    An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

    Published on: October 23, 2018

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    Related Experiment Videos

    Last Updated: Jun 20, 2026

    Direct Imaging of Laser-driven Ultrafast Molecular Rotation
    10:52

    Direct Imaging of Laser-driven Ultrafast Molecular Rotation

    Published on: February 4, 2017

    An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers
    09:49

    An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

    Published on: October 23, 2018

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    Area of Science:

    • Atomic, Molecular, and Optical Physics
    • Laser Spectroscopy
    • Quantum Optics

    Background:

    • Conventional laser spectroscopy often relies on intensity or polarization modulation.
    • Achieving Doppler- and background-free measurements typically requires intermodulation techniques.
    • Existing methods can be complex and may introduce artifacts.

    Purpose of the Study:

    • To introduce a new Doppler-free laser spectroscopy method.
    • To demonstrate the applicability of position modulation in laser spectroscopy.
    • To enable background-free fluorescence spectra recording without intermodulation.

    Main Methods:

    • Development of a Doppler-free laser spectroscopy technique utilizing laser beam position modulation.
    • Application of the technique in saturated absorption spectroscopy.
    • Demonstration in fluorescence measurements on sodium and iodine atomic lines.
    • Recording of Doppler- and background-free fluorescence spectra.

    Main Results:

    • Successful implementation of laser beam position modulation for Doppler-free spectroscopy.
    • Demonstrated applicability in both saturated absorption and fluorescence measurements.
    • Achieved background-free fluorescence spectra without intermodulation techniques.
    • Verified performance using sodium and iodine spectral lines.

    Conclusions:

    • Laser beam position modulation offers a new and effective approach to Doppler-free spectroscopy.
    • The technique simplifies background-free fluorescence measurements.
    • This method provides a valuable alternative for high-precision atomic and molecular spectroscopy.