Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

2.0K
This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
2.0K
Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

10.0K
In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
10.0K
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

14.5K
The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
14.5K
Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

9.2K
This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
9.2K
Primary Culture of Mouse Dopaminergic Neurons11:58

Primary Culture of Mouse Dopaminergic Neurons

39.1K
Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
39.1K
Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

26.4K
Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
26.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of human immunity to A/H3N2 influenza subclade K during 2025 emergence.

EBioMedicine·2026
Same author

Rapid cell culture-based assay for the detection of infectious viruses and viral vectors.

Journal of virological methods·2026
Same author

Impaired suppressive effect of FoxP3 regulatory T cells on B cells in multiple sclerosis.

Journal of neuroinflammation·2026
Same author

Comparative genomics identifies small interfering RNA with activity against all five human betacoronaviruses.

Genome medicine·2026
Same author

Identification of Viral Variants from Functional Genomics Data.

F1000Research·2026
Same author

Phosphorylation of SF3B1 by CDK11 orchestrates spliceosome activation via SNIP1-dependent RES complex recruitment.

Nature communications·2026
Same journal

RNF31 restricts EV-A71 replication through innate immune activation and VP4 degradation, and is antagonized by viral 3C proteases.

PLoS pathogens·2026
Same journal

How Saprolegniales became successful parasites.

PLoS pathogens·2026
Same journal

Anti-malarial contact dependent blocking of transmission of Plasmodium vivax by Anopheles darlingi mosquito vector.

PLoS pathogens·2026
Same journal

A single Citrobacter rodentium infection in Pink1 knockout and wild-type mice leads to regional blood-brain-barrier perturbation and limited microglial activation without dopamine neuron axon terminal loss.

PLoS pathogens·2026
Same journal

Correction: Structural basis for substrate recognition and inhibition of thioredoxin glutathione reductase from Schistosoma japonicum: Implications for antiparasitic development.

PLoS pathogens·2026
Same journal

Balancing under constraint: Structural insights into norovirus evolution and antigenic innovation.

PLoS pathogens·2026
See all related articles

Related Experiment Video

Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Published on: January 20, 2015

9.2K

Evolutionarily conserved herpesviral protein interaction networks.

Even Fossum1, Caroline C Friedel, Seesandra V Rajagopala

  • 1Max-von-Pettenkofer Institut, Ludwig-Maximilians-Universität, München, Germany.

Plos Pathogens
|September 5, 2009
PubMed
Summary
This summary is machine-generated.

This study maps protein interactions across multiple herpesviruses, revealing conserved interactions essential for viral function. Comparative analysis highlights conserved protein functions despite low sequence similarity, aiding in understanding viral biology.

More Related Videos

Primary Culture of Mouse Dopaminergic Neurons
11:58

Primary Culture of Mouse Dopaminergic Neurons

Published on: September 8, 2014

39.1K
Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

26.4K

Related Experiment Videos

Last Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Published on: January 20, 2015

9.2K
Primary Culture of Mouse Dopaminergic Neurons
11:58

Primary Culture of Mouse Dopaminergic Neurons

Published on: September 8, 2014

39.1K
Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

26.4K

Area of Science:

  • Virology
  • Molecular Biology
  • Bioinformatics

Background:

  • Herpesviruses are widespread DNA viruses causing diverse diseases in vertebrates.
  • Previous studies mapped protein interactions for Varicella-zoster virus (VZV) and Kaposi's sarcoma-associated herpesvirus (KSHV).

Purpose of the Study:

  • To systematically analyze protein interactions in three additional herpesvirus species: herpes simplex virus 1 (HSV-1), murine cytomegalovirus, and Epstein-Barr virus.
  • To perform a comparative analysis of protein interaction networks across all three herpesvirus subfamilies.

Main Methods:

  • Genome-wide yeast-two-hybrid (Y2H) screens were employed to identify protein interactions.
  • A total of 1,007 intraviral protein interactions were analyzed, combining new data with previously reported interactomes.

Main Results:

  • 735 novel protein interactions were identified through Y2H screens.
  • A core set of conserved protein interactions, such as HSV-1 UL33 with UL31/UL34, were identified.
  • Protein interactions were conserved between orthologous proteins, suggesting functional conservation over sequence similarity.

Conclusions:

  • Comparative interactome analysis enhances Y2H system coverage and reveals biologically relevant interactions.
  • Conserved protein interactions across herpesvirus species provide insights into fundamental viral mechanisms.