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Related Concept Videos

Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...

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Related Experiment Video

Updated: Jun 20, 2026

Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

Techniques to study autophagy in plants.

Géraldine Mitou1, Hikmet Budak, Devrim Gozuacik

  • 1Sabanci University, Orhanli, Tuzla, Istanbul, Turkey.

International Journal of Plant Genomics
|September 5, 2009
PubMed
Summary

Autophagy, a cellular recycling process, is crucial for plant growth, stress response, and pathogen defense. This review summarizes autophagy research and techniques applicable to plants.

Area of Science:

  • Plant Biology
  • Cellular Biology
  • Molecular Biology

Background:

  • Autophagy, a fundamental cellular recycling mechanism, has garnered significant research interest.
  • Studies in diverse model organisms reveal autophagy's role in various biological processes.
  • Evidence suggests autophagy is integral to plant growth, development, stress responses, and immunity.

Purpose of the Study:

  • To review general and plant-specific autophagy research.
  • To explain common techniques for studying autophagy.
  • To explore the adaptation of autophagy methodologies from other organisms to plant studies.

Main Methods:

  • Literature review of autophagy research in yeast, mammals, and plants.
  • Summary of established molecular and cellular techniques for autophagy analysis.

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Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
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Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
09:59

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

Related Experiment Videos

Last Updated: Jun 20, 2026

Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
09:59

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

  • Discussion of potential cross-species application of these techniques in plant science.

    • Main Results:

    • Autophagy plays a multifaceted role in plant life cycles and environmental interactions.
    • A range of molecular techniques are available for studying autophagy.
    • Existing methods in model organisms can likely be adapted for plant autophagy research.

    Conclusions:

    • Autophagy is a vital process in plants with implications for agriculture and stress resilience.
    • Further development and adaptation of techniques will enhance our understanding of plant autophagy.
    • Cross-disciplinary approaches can accelerate discoveries in plant autophagy research.